Fundamentals of widefield and confocal microscopy and imaging

Application deadline: 9 February 2024. This course will provide the participants with an overview of the basics of microscopy (light and optics, contrast techniques and imaging). The confocal part will include multicolour, 3D, and time-lapse imaging as well as spectral imaging, introduction to photobleaching and photoactivation techniques. Basic image analysis tools including measurement functions, colocalisation, and spectral unmixing as well as 3D/4D image processing will be covered, both in theory and practice. The course will combine lectures with intense daily hands-on practices followed by discussions.